Researchers Find Loss Of Lipid-Regulating Gene Fuels Prostate Cancer Spread
In a new study, researchers have identified a lipid-regulating protein that conveys what they describe as "superpowers" onto prostate cancer cells, causing them to aggressively spread.
It was conducted by researchers of Johns Hopkins Kimmel Cancer Center from the Department of Radiation Oncology and Molecular Radiation Sciences and its findings were reported in the journal Molecular Cancer Research.
In studies of human prostate cancer cell and stromal cell lines, when the lipid-regulating protein, called CAVIN1, was removed from stromal cells -- the connective tissue cells in and around tumours -- the cells no longer used the lipids. Instead, cancer cells feasted on lipids in the environment, using them as fuel, including to make hormones that feed cancer.
"We know that aggressive behaviour of tumours, such as rapid growth and metastasis, does not happen alone, so we wanted to find out the role of the tumour microenvironment, and specifically the interaction between prostate tumour cells and stromal cells, in increasing cancer growth," says Marikki Laiho, MD, PhD, director of the Division of Molecular Radiation Sciences and professor of radiation oncology and molecular radiation sciences at the Johns Hopkins University School of Medicine, and senior author of the study.
In the human cell line experiments, when the researchers took CAVIN1 away from stromal cells called fibroblasts, the stromal cells no longer used the lipids, but the lipids remained in the environment, and to the researchers' surprise, they became a smorgasbord for the cancer cells. In every prostate cancer cell line tested, tumour cells universally had an appetite for the lipids, using them to fuel growth, strengthen the protective membrane around the cell, synthesize proteins and make testosterone to support cancer's growth, says Jin-Yih Low, PhD, first author and a research fellow in the Department of Radiation Oncology and Molecular Radiation Sciences.
"As a consequence, the tumour cells behaved more aggressively, exhibiting invasive and metastatic behaviour," says Low. "Just having access to the lipids gave the tumour cells more power. The tumour is the same tumour, but the behaviour of the tumour changes."
In addition, when the stromal fibroblasts did not use the lipids, they changed and started to secrete inflammatory molecules that altered the tumour microenvironment. Inflammation is a characteristic long known to promote cancer.
To confirm their findings, the researchers conducted similar experiments in mouse models, implanting the prostate cancer cells and stromal cells into the prostates of mice and comparing the behaviour of tumours with and without CAVIN1 function in the stromal cells. Although the presence or loss of CAVIN1 did not impact the speed of tumour growth, lack of CAVIN1 caused cancer to spread. All of the mice with tumours that did not express CAVIN1 had a twofold to fivefold increase in metastasis. The tumours also had a fortyfold to a hundredfold increase in lipids and inflammatory cells.
Laiho calls the findings striking. "We suspected CAVIN1 was important but did not realize how important," she says. "Microenvironment matters and the amount of lipids matters a lot. Just by converting the cells from a low level of lipids to a high level of lipids created a situation of rampant metastasis."
The researchers say the loss of CAVIN1 in tumour cells could potentially be used as a biomarker, alerting clinicians to a risk of metastasis. Interventions are being studied but are challenging because all cells need lipids. Any treatments aimed at inhibiting lipids would have to specifically target cancer cells. Ongoing studies are aimed at better understanding the inflammatory process and ways to stop its ability to fuel cancer spread. For example, the researchers want to understand why the inflammation draws cells, called macrophages, that further exacerbate the inflammatory process but does not attract beneficial T cells that could attack cancer, and if lipid cells could be sending signals that affect immune checkpoints, which are the immune systems on and off switches.
CAVIN1 was first discovered in human lipodystrophy syndrome, a disorder that prevents the uptake of lipids and leaves those affected unable to make fat cells, placing them at risk of diseases including cardiovascular disease and diabetes.
(ANI)
